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Dr. Pradeep Kumar Chand

Pradeep Kumar Chand Name : Pradeep Kumar Chand
Designation : Professor of Botany & Director, UGC-Human Resource Development Centre
Qualification : Ph.D. (Nottingham, UK)
Phone No. : +91-8895300135
E-mail ID : pkchanduubot(at)rediffmail(dot)com

  • Plant Biotechnology & Genetic Engineering
    • Plant Cell, Tissue & Organ culture
    • Protoplast Fusion for Somatic Hybridization
    • Agrobacterium–mediated Plant Genetic Transformation
    • Direct Gene Transfer to Plants (PEG, Electroporation, Biolistic)
    • Sense & Antisense Gene Cloning
    • DNA Fingerprinting for Estimation of Genetic Diversity

  • Cytogenetics
  • Cell & Molecular Biology
  • Plant Biotechnology
  • D.Sc
    • Awarded: 01
  • Ph.D.
    • Awarded: 15
    • Thesis Submitted: 01
    • Continuing: 04
  • M.Phil.
    • Awarded: More than 25
1 Rech, E. L., Ochatt, S.J., Chand, P.K. ,Power,J.B. and Davey, M.R. : Electro-enhancement of plant protoplast derived cell division. Protoplasma 141: 169-176 (1987).
2 Chand, P.K., Davey, M.R., Power,J.B. and Cocking, E.C. : An improved procedure for protoplast fusion using polyethylene glycol. Journal of Plant Physiology 133 : 480-485 (1988).
3 Ochatt, S.J., Chand, P.K., Rech, E. L., Davey, M.R. and Power, J.B : Electroporation-mediated improvement of plant regeneration from colt cherry (Prunus avium x Pseudocerasus) protoplasts. Plant Science 54 : 165-169 (1988).
4 Chand, P.K., Ochatt, S.J., Rech, E. L. ,Power,J.B. and Davey, M.R. : Electroporation stimulates plant regeneration from protoplasts of the woody medicinal species Solanum dulcamara L. Journal of Experimental Botany 39 : 1267-1276 (1988).
5 Rech, E. L., Ochatt, S, J., Chand, P.K., Davey, M. R., Mulligan, B. J. and Power, J.B. : Electroporation increases DNA synthesis in cultured plant protoplasts. Nature Biotechnology 6 : 1091-1093 (1988).
6 Chand, P.K., Rech, E. L., Golds, T. J., Power,J. B. and Davey, M.R. : Electroporation stimulates transformation of freshly isolated protoplasts of Solanum dulcamara L. by Agrobacterium. Plant Cell Reports 8 : 86-89 (1989).
7 Aziz, M. A., Chand, P.K., Power,J. B. and Davey, M.R. : Somatic hybrids between the forage legumes Lotus corniculatus L. and L. tenuis Waldst et Kit. Journal of Experimental Botany 41 : 471-479 (1990).
8 Chand, P. K., Davey, M.R., and Power, J. B. : Efficient plant regeneration from cell suspension protoplasts of the woody medicinal plant Solanum dulcamara L. (bittersweet, woody night shade). Plant Cell Tissue Organ Culture 22 : 119-125 (1990).
9 Chand, P.K., Davey, M.R., Power,J.B. and Cocking, E.C. : Amphidiploid somatic hybrids between Solanum dulcamara L. and Solanum viarum Dunal by micromanipulation of heterokaryons following protoplast electrofusion. The Nucleus 30 : 53-58 (1991).
10 Pattnaik, S.K., Sahoo, Y. and Chand, P.K.: Efficient plant retrieval from alginate-encapsulated vegetative buds of mature mulberry trees. Scientia Horticulturae 61: 227-239 (1995).
11 Chand, P.K. and Chand, S. : High frequency plant regeneration from Solanum viarum protoplasts. Journal of Herbs, Spices & Medicinal Plants (USA) 3(4): 21-34 (1995).
12 Dwari, M. and Chand, P.K. : Evaluation of explants, growth regulators and culture passage for enhanced callus induction, proliferation and plant regeneration in the tree legume Dalbergia lanceolaria L. Phytomorphology 46(2): 123-131 (1996).
13 Pattnaik, S.K. and Chand, P.K. : In vitro propagation of two medicinal herbs, Ocimum americanum L. syn. O. canum Sims. (hoary basil) and O. sanctum L. (holy basil). Plant Cell Reports 15: 846-850 (1996).
14 Pattnaik, S.K., Sahoo, Y. and Chand, P.K. : Micropropagation of a fruit tree, Morus australis Poir. syn. M. acidosa Griff. Plant Cell Reports 15: 841-845 (1996).
15 Chand, P.K., Sahoo, Y., Pattnaik, S.K. and Patnaik, S.N. : Evaluation of procedures for interspecific protoplast fusion in mulberry. Indian Journal of Sericulture 35(1): 46-49 (1996).
16 Sahoo, Y., Pattnaik, S.K. and Chand, P.K. : Plant regeneration from callus cultures of Morus indica L. derived from seedling and mature plants. Scientia Horticulturae 69: 85-98 (1997).
17 Bhuyan, A.K., Pattnaik, S.K. and Chand, P.K. : Micropropagation of curry leaf tree (Murraya koenigii (L) Spreng.) by axillary proliferation using intact seedlings. Plant Cell Reports 16: 779-782 (1997).
18 Sahoo, Y., Pattnaik, S.K. and Chand, P.K. : In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (Sweet basil) by axillary shoot proliferation. In Vitro Cellular & Developmental Biology - Plant 33: 293-296 (1997).
19 Pattnaik, S.K. and Chand, P.K.: Rapid clonal propagation of three important mulberries : Morus cathyana Hemsl., M. lhou Koiz. and M. serrata Roxb. through in vitro culture of apical shoot buds and nodal explants from mature trees. Plant Cell Reports 16: 503-508 (1997).
20 Pradhan, C., Pattnaik, S.K., Dwari, M., Patnaik, S.N. and Chand, P.K.: Efficient plant regeneration from cell suspension derived callus of East Indian Rosewood (Dalbergia latifolia Roxb.). Plant Cell Reports 18: 138-142 (1998).
21 Sahoo, Y. and Chand, P.K. : In vitro multiplication of a medicinal herb, Tridax procumbens L. (Mexican Daisy, Coatbuttons) : Influence of explanting season, growth regulator synergy, culture passage and planting substrate. Phytomorphology 48: 195-205 (1998).
22 Nayak, N.R., Chand, P.K., Rath, S.P. and Patnaik, S.N. : Influence of some plant growth regulators on the growth and organogenesis of Cymbidium aloifolium (L.) Sw. seed-derived rhizomes in vitro. In Vitro Cellular & Developmental Biology - Plant 34: 185-188 (1998).
23 Pradhan, C., Kar, S., Pattnaik, S.K. and Chand, P.K. : Propagation of Dalbergia sissoo Roxb. through in vitro shoot proliferation from cotyledonary nodes. Plant Cell Reports 18: 122-126 (1998).
24 Pradhan, C., Pattnaik, S.K. and Chand, P.K. : Rapid in vitro propagation of East Indian Rosewood (Dalbergia latifolia Roxb.) through high frequency shoot proliferation from cotyledonary nodes. Journal of Plant Biochemistry and Biotechnology 7 : 61-64 (1998).
25 Sahoo, Y. and Chand, P.K. : Micropropagation of Vitex negundo L. a woody aromatic medicinal shrub through high frequency axillary shoot proliferation. Plant Cell Reports 18: 301-307 (1999).
26 Naik, S.K., Pattnaik, S.K. and Chand, P.K. : In vitro propagation of pomegranate (Punica granatum L. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature tree. Scientia Horticulturae 79: 175-183 (1999).
27 Naik, S.K., Pattnaik, S. and Chand, P.K. : High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate (Punica granatum L.). Scientia Horticulturae 85: 261-270 (2000).
28 Mandal, J., Pattnaik, S. and Chand, P.K : Alginate encapsulation of axillary buds of Ocimum americanum L. (hoary basil), O. basilicum L. (sweet basil), O.gratissimum L. (shrubby basil) and O. sanctum L. (sacred basil). In Vitro Cellular & Developmental Biology- Plant 36 (4) : 287-292 (2000).
29 Pattnaik, S., and Chand, P.K. : Morphogenic response of the alginate - encapsulated axillary buds from in vitro shoot cultures of six mulberries. Plant Cell Tissue Organ Culture 60: 177-185 (2000).
30 Pattnaik, S., Pradhan, C., Naik, S.K. and Chand, P.K. : Shoot organogenesis and plant regeneration from hypocotyl - derived cell suspensions of a tree legume Dalbergia sissoo Roxb. In Vitro Cellular & Developmental Biology - Plant 36(5): 407-411 (2000).
31 Mandal, J., Pattnaik, S. and Chand, P.K. : Growth response of the alginate -encapsulated nodal segments of in vitro - cultured basils. Journal of Herbs, Spices & Medicinal Plants 8(4): 65-74 (2001).
32 Mondal, T.K., Bhattacharya, A., Ahuja, P.S. and Chand, P.K. : Transgenic tea [Camellia sinensis (L.) O. Kuntze cv. Kangra Jat] plants obtained by Agrobacterium - mediated transformation of somatic embryos. Plant Cell Reports 20: 712-720 (2001).
33 Singh, A.K., Chand, S., Pattnaik, S. and Chand, P.K.: Adventitious shoot organogenesis and plant regeneration from cotyledons of Dalbergia sissoo Roxb., a timber yielding tree legume. Plant Cell, Tissue and Organ Culture 68: 203-209 (2002).
34 Mondal, T.K. and Chand, P.K. : Detection of genetic variation among micropropagated tea [Camellia sinensis (L.) O. Kuntze] by RAPD analysis. In vitro Cellular & Developmental Biology - Plant 38(3): 296-299 (2002).
35 Naik, S. and Chand, P.K. : Silver nitrate and aminoethoxyvinylglycine promote in vitro adventitious shoot regeneration of pomegranate (Punica granatum). Journal of Plant Physiology 160 (4) : 423- 430 (2003).
36 Barik, D. P., Naik, S.K., Mohapatra, U. and Chand, P.K. : High frequency axillary shoot proliferation in cotyledonary node explants of grasspea (Lathyrus sativus L.). In vitro Cellular & Developmental Biology ‚?" Plant 40 (5) : 467-470 (2004).
37 Barik, D.P., Mohapatra, U. and Chand, P.K. : High frequency in vitro regeneration of Lathyrus sativus L. Biologia Plantarum 49 : 637-639 (2005).
38 Barik, D.P., Mohapatra, U. and Chand, P.K. : Transgenic grass pea (Lathyrus sativus L.): Factors influencing Agrobacterium ‚?" mediated transformation and regeneration. Plant Cell Reports 24 : 523-531 (2005).
39 Barik, D.P., Mohapatra, U. and Chand, P.K. : Direct adventitious shoot organogenesis in epicotyl segments of grasspea (Lathyrus sativus L.). Australian Journal of Botany 54: 505-508 (2006).
40 Naik, S.K. and Chand, P.K. : Nutrient-alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Scientia Horticulturae 108 : 247-252 (2006).
41 Barik, D. P., Naik, S.K., Mudgal, A. and Chand, P.K. : Rapid plant regeneration through in vitro axillary shoot proliferation of butterfly pea (Clitoria ternatea L.) ‚?" a twinning legume. In vitro Cellular & Developmental Biology ‚?" Plant 43 : 144-148 (2007).
42 Barik, D. P., Acharya, L., Mukherjee, A. K., Chand, P.K. Analysis of genetic diversity among the selected grasspea (Lathyrus sativus L.) genotypes using RAPD markers. Zeitscrift fur Naturforschong 62c: 869-874 (2007).
43 Satapathy, K.B. and Chand, P.K.: Growth and nitrogen content of symbiont-free waterfern Azolla regenerated from in vitro culture of frond primordia. Cytologia 73(4): 445-452 (2008).
44 Nayak, P., Behera, P.R., Thirunavoukkarasu, M. and Chand, P.K. : High frequency plant regeneration through adventitious multiple shoot organogenesis in epicotyl explants of Indian gooseberry (Emblica officinalis Gaertn.) Scientia Horticulturae 123: 473-478 (2010).
45 Behera, P.R., Nayak, P., Barik, D.P., Rautray, T. R.,.Thirunavoukkarasu, M., and Chand, P.K.: ED-XRF spectrometric analysis of comparative elemental composition of in vivo and in vitro roots of Andrographis paniculata (Burm.f.) Wall.exNees - a multi-medicinal herb. Applied Radiation and Isotopes 68 :2229-2236 (2010).
46 Swain, S.S, Tripathy, T., Mohapatra, P.K. and. and Chand, P.K. : Photosynthetic and transpiration responses of in vitro regenerated Solanum nigrum L.. during ex vitro adaptation. In Vitro Cellular & Developmental Biology- Plant. 46:134‚?"141(2010).
47 Swain S.S., Sahu, L., Barik, D. and Chand, P.K. Agrobacterium x plant factors influencing transformation of Joseph’s Coat (Amaranthus tricolor L.). Scientia Horticulturae 125 (3) : 461-468 (2010).
48 Jena, R. C., Samal, K.C., Chand, P.K., Das, B.K. : Molecular characterization of 12 mango germplasm using RAPD markers. Plant Tissue Culture & Biotechnology 20(1): 91-99 (2010).
49 Naik, S.K. and Chand, P.K.: Tissue culture-mediated biotechnological intervention in pomegranate - a review. Plant Cell Reports 30 : 707-721 (2011).
50 Nayak, P., Behera, P.R.,Thirunavoukkarasu, M., and Chand, P.K.: ED-XRF spectrometry-based comparative inorganic profile of leaf-derived in vitro calli and in vivo leaf samples of Phyllanthus amarus Schum. & Thonn. ‚?" a hepatoprotective herb. Applied Radiation and Isotopes 69 :567-573 (2011).
51 Das, A.B, Jena, S., Pradhan, C. and Chand, P.K. : Genetic variability among male populations of a minor mangrove Excoecaria agallocha L. as evident by chromosome morphology and DNA markers. Nucleus 54(1):39‚?"47 (2011).
52 Swain, S.S., Ray, D.K., Chand, P.K. ED-XRF spectrometry-based trace element composition of genetically engineered rhizoclones vis-√†-vis natural roots of a multi-medicinal plant, butterfly pea (Clitoria ternatea L.). Journal of Radioanalytical and Nuclear Chemistry 293:443‚?"453 (2012).
53 Swain, S.S., Rout, K.K., Chand, P.K. Production of triterpenoid anti-cancer compound taraxerol in Agrobacterium-Transformed Root Cultures of butterfly pea (Clitoria ternatea L.). Applied Biochemistry and Biotechnology 168:487‚?"503 (2012).
54 Swain S.S., Sahu, L., Pal, A., Barik, D. , Pradhan, C. and Chand, P.K. Hairy root cultures of butterfly pea (Clitoria ternatea L. ) : Agrobacterium x plant factors influencing transformation. World Journal of Microbiology & Biotechnology (2012) 28:729‚?"739.
55 Samal, K.C., Jena, R. C., Swain, S.S., Das, B.K., Chand, P.K. : Evaluation of genetic diversity among commercial cultivars, hybrids and local mango (Mangifera indica L.) genotypes of India using cumulative RAPD and ISSR markers. Euphytica (2012) 185:195‚?"213.
56 Mondal, T.K., Bantwa P., Sarkar, B., Ghosh, P. and Chand, P.K. : Cellular differentiation, regeneration, and secondary metabolite production in medicinal Picrorhiza spp. Plant Cell Tissue and Organ Culture 112:143‚?"158 (2013).
57 Behera, P.R., Thirunavoukkarasu, M. and Chand, P.K.: Adventitious plant regeneration from different explants of Aegle marmelos (L.) Corr. Journal of Medicinal Plant Research 7 (37): 2761-2768 (2013).
58 Sahu, L., Jena, S.S., Swain, S.S., Sahoo, S., Chand, P.K. Agrobacterium rhizogenes -mediated transformation of a multi-medicinal herb,  Boerhaavia diffusa L.: Optimization of the process and anti-microbial activity against bacterial pathogens causing urinary tract infections. Frontiers in Life Sciences 7(3-4): 197-209 (2013).
59 Pal, A., Swain, S.S., Mukherjee,,A.K., Das A. B. and Chand, P.K. Stable germ line transformation of a leafy vegetable crop amaranth (Amaranthus tricolor L.) mediated by Agrobacterium tumefaciens. In Vitro Cellular & Developmental Biology- Plant 49: 114-128 (2013).
60 Pal, A., Swain, S.S., Mukherjee,,A.K., Chand, P.K. Agrobacterium pRi TL-DNA rolB & TR-DNA opine genes transferred to the spiny amaranth (Amaranthus spinosus L.) ‚?"A nutraceutical crop. Food Technology & Biotechnology 51 (1): 26-35 (2013).
61 Behera, P. R., Parhi, P., Roy, D. K., Rautray, T. R., Pal, A., Thirunavoukkarasu, M., Chand, P. K. Multi-elemental profiling of in vitro calliclones vis-√†-vis in vitro and in vivo leaf samples of a medicinal herb Hybanthus enneaspermus (L.) F. Muell. using energy dispersive X-ray fluorescence technique. Advanced Science Letters 20 (3-4): 769-777(2014).
62 Sahu, L., Ray. D. K., Chand, P.K. Proton induced X-ray emission (PIXE) technique for determining multi-element composition of transformed hairy root cultures of Boerhaavia diffusa L.: an important medicinal herb. Journal of Radioanalytical and Nuclear Chemistry 300:345‚?"354 (2014).
63 Rout K.K., Swain S.S., Chand P.K.: Quantification of ő≤-sitosterol in hairy root cultures and natural plant parts of butterfly pea (Clitoria ternatea L.). Journal of Planar Chromatography-Modern TLC 27 (1): 42‚?"46 (2014).
64 Jena, S.S., Sahu, L., Ray, D. K., Mishra, S. K., Chand, P.K.: PIXE - based quantification of health-proactive trace elements in genetically transformed roots of a multi-medicinal plant, Sida acuta Burm.f. Journal of Radioanalytical and Nuclear Chemistry 303:2101-2112 (2015).
65 Nayak, P., Sharma, M., Behera, S. N., Thirunavoukkarasu, M. and Chand, P.K.: High-performance liquid chromatographic quantification of plumbagin from transformed rhizoclones of Plumbago zeylanica L.: Inter-clonal variation in biomass growth and plumbagin production. Applied Biochemistry and Biotechnology 175:1745-1770 (2015).
66 Mukhopadhyay, M., Mondal, T. K., Chand, P.K.: Biotechnological advances in tea (Camellia sinensis [L.] O. Kuntze) ‚?" a review. Plant Cell Reports. 35: 255-287 (2016).
67 Jena, R.C., Samal, K.C., Pal, A., Das, B. K., Chand, P.K.: Genetic diversity among some promising Indian local selections and hybrids of cashew nut based on morphometric and molecular markers. International Journal of Fruit Science 16: 69‚?"93 (2016).
68 Behera, P. R. Jena, R. C., Das, A., Thirunavoukkarasu M., Chand, P.K.: Genetic stability and coumarin content of transformed rhizoclones and regenerated plants of a multi-medicinal herb, Hybanthus enneaspermus (L.) F. Muell. Plant Growth Regulation DOI: 10.1007/s10725-016-0145-3 (2016) (published online 14 January 2016).
69 Chand, P. K. : Enzyme purification improves protoplast plating efficiency of the woody medicinal species Solanum dulcamara L. Plant Science Research 121 : 43‚?"45 (1990).
70 Chand, P.K., Sahoo,Y. and Pattnaik, S. K. : Artificial seeds ‚?" A novel method for mulberry propagation. Indian Silk 30 : 33-38 (1994).
71 Tripathy, S. K., Swain, P.K., Patnaik, S. N., Chand, P. K. and Kole, C.R. : Optimization of variables for effective tissue culture of Lathyrus sativus L. Orissa Journal of Agricultural Research 7 : 26-29 (1994).
72 Chand, P.K., Sahoo,Y. and Pattnaik, S. K. : Prospects of in vitro genetic manipulations in mulberry sericulture. Indian Silk 33 : 3-6 (1994).
73 Patnaik, S. N. and Chand, P. K. : Protoplast techniques for plant genetic engineering. Plant Science Research 18 : 77-92(1996).
74 Chand, P.K., Sahoo, Y., Pattnaik, S.K. and Patnaik, S.N. : Evaluation of procedures for interspecific protoplast fusion in mulberry. Indian Journal of Sericulture 35(1): 46-49 (1996).
75 Chand, P. K. and Pattnaik, S. K. : Variation in morphogenic response of eight elite mulberry (Morus species) genotypes in vitro. Development Science 1: 51-68 (1998).
76 Barik, D.P., Mohapatra, S., Mohapatra, U. and Chand, P.K. : Callus induction and plant regeneration in grasspea, Lathyrus sativus L. cv. `Nayagarh Local'. Plant Science Research 23: 10-16 (2001).
77 Chand, P.K. : Uptake of a membrane-impermeable dye phenosafranine by electroporated protoplasts of a medicinal plant species Solanum dulcamara L. Plant Science Research 26: 10-15(2004).
78 Beura, R., Mohanty, I., Sahoo, D., Mahapatra, D. and Chand, P.K. : Direct ex vitro tuber formation in minitubers of potato (Solanum tuberosum L.) raised through in vitro culture. Plant Science Research 27 : 81-88 (2005).
79 Beura, R., Mahapatra, D. and Chand, P.K. : In vitro microtuberization for production of seed potato (Solanum tuberosum L.). Proceedings of AP Akademi of Sciences 9 (2) : 253-258 (2005).
80 Naik, S.K. and Chand, P.K.: Micropropagation of pomegranate through in vitro culture of meristems : a review. Plant Science Research 32 : 9-17 (2010).
81 Swain, S.S., Pal, A., Sahu, L., Pradhan, C., Barik, D.P., Chand, P.K.: Nutrient-alginate encapsulated nodal explants of butterfly pea (Clitoria ternatea L.) as synseeds for germplasm conservation and exchange. Plant Science Research 32: 48-55 (2010).
82 Mukherjee, A., Naskar, S. K., Poddar, A., Dasgupta, M., Sahoo, M.R. and Chand, P.K. : Submergence and salinity tolerance in sweet potato (Ipomoea batatas L.) Progress and prospects. Plant Science Research 31: 1-9 (2010).

83 Behera, P.R., Pal, A., Thirunavoukkarasu, M. and Chand, P.K. : Elemental profiling of leafy shoots of in vitro regenerated plants of a rare multi-medicinal herb Hybanthus enneaspermus (L.) F. Muell. : Implications in human healthcare. Plant Science Research 33: 34-43 (2011).
84 Mukherjee, A., Naskar, S.K., Pradhan, D.M.P., Sahoo, B.K., Sahoo, M.R., Mukherjee, A.K. and Chand, P.K. : Biotic and abiotic stress tolerance in taro (Colocasia esculenta [L.] Schott.) for adaptability to changing climate : A review. Plant Science Research 33: 1-10 (2011).
85 Nayak, P., Thirunavoukkarasu, M. and Chand, P.K.: An optimized protocol for efficient in vitro propagation of white leadwort (Plumbago zeylanica L.) ‚?" an important medicinal shrub. Plant Science Research 33: 44-51(2011).
86 Sahu, L., Swain, S.S., Pal,A., and Chand, P.K. : Acetosyringone stimulates hairy root formation in an important ethno-medicinal plant species, Boerhaavia diffusa L. via Agrobacterium rhizogenes‚?"mediated genetic transformation. Plant Science Research 33: 17-26 (2011).

Chand, P. K. and Aziz, M.A. : Genetic engineering of some crop species using Ri plasmid vectors. In : Proceedings National Symposium : “Plant Sciences in the Nineties”, Kalyani University, India pp. 114-126 (1991).
Rao, M. S., Swain, P.K., Chand, P.K., Patnaik, S. N. and Rath, S. P. : Towards micropropagation of Pterocarpus marsupium Roxb. Proceedings of Third All India Conference of Cytology and Genetics, Nagpur, India Vol. 3 pp. 96-100 (1992).
Aziz, M. A., Chand, P. K. and Davey, M. R. : Transformation of Lotus species using an Ri plasmid vector. In : Moss, J. P. (ed.) “Biotechnology and Crop Improvement in Asia”, ICRISAT, Patancheru, A. P., India. pp. 201-203 (1992).
Pattnaik, S.K. and Chand, P.K. : Artificial seeds as an aid to clone white mulberry (Morus alba L.). In : Dautzenberg, H. and Poncelet, D. (eds) Proceedings International Workshop “Bioencapsulation V. From Fundamentals to Industrial Applications”, Potsdam, Germany pp. 165-168 (1996).
Satapathy, K. B., Chand, P. K. and Mohanty, R. C. : Symbiotic cyanobacterium Anabaena azollae and organic rice farming. In : Mohanty, R. C. and Chand, P. K. (Eds.) “Microbes in our Lives”, PGAIM, Utkal University, pp.26-46 (2005).
Satapathy, K. B., Chand, P. K. and Mohanty, R. C. : Azolla biofertilizer for rice farming : an appraisal. In : Panigrahi, J. K. (Ed.) “Science for Society”. ISCA : Bhubaneswar, pp. 150-163 (2005).
Satapathy, K.B. and Chand, P.K. : Some familiar ethno-medicinal plants of Orissa used in homeopathy. In: Proc Natl. Sem. “Common Medicinal Plants and Their Use”, Jan 8, 2008, pp. 21-29 (2008).
Satpathy, K.B. and Chand, P.K. : Dwindling medicinal plant diversity in medium and low land rice fields of coastal Orissa. In: Gupta, N. and Mohapatra, A.K. (Eds.) Proc. Symp. “Wetland and Mangrove Biodiversity in Orissa Coast”, Regional Plant Resource Centre, Bhubaneswar, pp. 17-20 (2008).
Chand, P. K., Sahoo, S. and Satapathy, K. B. : Tissue culture studies on some important medicinal plants of Orissa. In : Sahoo, R. K. (ed.) Conservation, Cultivation and Utilization of Medicinal Plants. (2008).
Chand, P. K., Mohapatra, U. and Rath, S.P.: Towards engineering herbicide tolerance in black gram (Vigna mungo [L.] Hepper). In : Patra, H. K. (ed.) “Abiotic Stress Tolerance”, SAP-DRS in Botany, Utkal University, Bhubaneswar pp. 38-54 (2008).
Poddar, A., Mohapatra, P.K. and Chand, P.K. : Nickel effects on seedling physiology of eggplant (Solanum melongena L.). In: H.K. Patra (Ed.) “Attenuation of Stress Impacts on Plants”, SAP-DRS in Botany, Utkal University, Bhubaneswar pp. 109-116. (2009).
Satapathy, K.B. and Chand, P.K. : Phytoremediation of chromium toxicity in water resources of ‘Sukinda chrome zone’ using Azolla and its floating associates. In: Bastia, A.K. and Mohapatra, U.B. (Eds.) Proc. Natl. Sem. “Recent Trends in Monitoring and Bioremediation of Mine and Industrial Environment” North Orissa University, Baripada, pp.54-62 (2009).
Barik, D.P., Mohapatra, U.B. and Chand, P.K. : Agrobacterium ‚??mediated transformation of grass pea. In: Vaz Patto, C. and Rubiales, D. (Eds.) Grain legumes 54: 14 (2009).
Chand, P.K. : Frontiers in life sciences. Key note address. In: Natl. Conf. “New Frontiers in Life Sciences”, XIII Odisha Bigyan Congress, ISCA, Dec 09-11: pp.01-35 (2010).
Chand, P.K., Cocking, E.C., Lister, A., Davey, M. R. and Power,J.B. :¬† An improved PEG fusion procedure for flow sorting of plant protoplasts. In : Puite et al (eds.) “Progress in Plant Protoplast Research”, Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 211-212 (1988).

Convener: National Seminar on ‚??‚??Biotechnological Approaches for Conservation & Utilization of Medicinal & Aromatic Plants‚?Ě on the 25th March 2014‚?Ě.
Convenor-cum-Organizing Secretary: 7th Odisha Bigyan Congress, 2003.

1 Documentation of epiphytic and epilithic lichen biodiversity of Kapilash and other protected forests of Dhenkanal District of Odisha. (Funding Agency: Odisha Biodiversity Board (OBB), Govt. of Odisha)
2 An impact assessment study on flora and fauna biodiversity due to buxite mining project at Panchapatmalli Central & North Block ML, Damanjodi. (Funding Agency: NALCO)
3 Molecular screening of mungbean (Vigna radiata [L.] Wilczek) germplasm of Orissa for biotic and abiotic stress tolerance. (Funding Agency: University Grants Commission, New Delhi)
4 Production & Utilisation of Agrobacterium-Transformed Hairy Root Cultures of Some Important Medicinal Plants of Orissa as a Source of Pharmaceutical Compounds. (Funding Agency: Government of Orissa Science & Technology Department)
5 Multi-elemental and secondary metabolite characterization of transformed hairy root cultures of some potential anti-diabetic plants of Orissa. (Funding Agency: University Grants Commission, New Delhi)
6 Suppression of the neurotoxic amino acid in seed storage protein of Lathyrus sativus L. via mutation techniques and gene transfer. (Funding Agency: International Atomic Energy Agency (I.A.E.A.), Vienna, Austria)
7 Production of interspecific Azolla hybrids by protoplast fusion for utilization in rice cultivation in adverse eco-geographic locations of Orissa. (Funding Agency: Indian Council of Agricultural Research (I.C.A.R))
8 Transfer of salt tolerance to the timber ‚??yielding forest tree legume species of Dalbergia (D. latifolia and D. sissoo) via interspecific protoplast fusion with a mangrove associate, D. spinosa. (Funding Agency: Department of Biotechnology (DBT) Govt. of India)
9 Production and utilization of artificial seeds for mulberry propagation (Extension of Project-I). (Funding Agency: Central Silk Board, Ministry of Textiles, Govt. of India)
10 Genetic improvement of mulberry by protoplast culture and fusion. (Funding Agency: Central Silk Board, Ministry of Textiles, Govt. of India)

1 Shyamananda Pattnaik Memorial Gold Medal as Best Graduate in Botany (Hons) of Utkal University in 1976.
2 University Gold Medal for securing First Rank in First Class in M.Sc. Examination in Botany of Utkal University in 1978.
3 IRRI Research Fellowship: at the International Rice Research Institute, Manila, The Philippines : 1985.
4 Commonwealth Scholarship by the British Council for conducting doctoral research (Ph.D.): at the University of Nottingham, UK : 1985 - 1988.
5 SERC (Science & Engineering Research Council, Dept. of Science and Technology, Govt. of India) Visiting Fellowship: at University of Delhi South Campus, New Delhi : 1997-98.
6 INSA (Indian National Science Academy) Visiting Fellowship: University of Delhi South Campus, New Delhi : 1998-99.
7 Biotechnology Overseas Associateship (Department of Biotechnology, Govt. of India): University of New Hampshire, Durham, USA : 1999 - 2000.
8 Bijoy Govind Memorial Gold Medal - 2003 for contribution to the field of Plant Biotechnology & Genetic Engineering in the State of Odisha.
9 “UGC Research Award” (University Grants Commission, New Delhi) : 2006.
10 Fellow, Indian Botanical Society (FBS): Since October, 1995.
12 Member, The National Academy of Sciences, India (MNASc) : Since April, 1998.
13 OUAT Award for Significant Contribution to Botany: conferred by Orissa Botanical Society: 2014.
14 President, Orissa Botanical Society: 2015.
15 Member of the Syndicate, Utkal University: since 2015.
11 Fellow, Society of Applied Biotechnology (FSAB): Since March, 2012.

  • Teaching: 36 years (1979-2015)
  • Research: 35 years (1980-2015)
  • Institution Period Designation
      From To  
    Post Graduate Dept of Botany, Utkal University, Vani Vihar October 24, 1979 Continuing Lecturer(1979), Reader (1992), Professor (2000)
    School of Biological Sciences, University of Nottingham, England, UK October, 1984 September, 1985 Research Fellow (E.E.C. Project)

    International Rice Research Institute (I.R.R.I.), Manila,

    The Philippines

    October 4, 1985 December 31, 1985 Research Fellow (Soil Microbiology Division)
    Plant Genetic Manipulation Group, University of Nottingham, England, UK January, 1986 December 1988 PhD Scholar (Graduate student)
    Department of Genetics University of Delhi South Campus, New Delhi September,12 1998 December,12 1998 INSA Visiting Fellow (Indian National Science Academy)
    Department of Genetics University of Delhi South Campus, New Delhi January 5, 1999 March 31, 1999 SERC Visiting Fellow (DST, Govt. of India)
    Department of Plant Biology University of New Hampshire Durham, NH 03824, USA September 1, 2000 May 31, 2001 Biotechnology Overseas Associate (DBT, Govt. of India)
  • Important Activities:
    • Collaborative research conducted at Utkal University (1989-1997, 2001-2014)
      • Purification, characterization (IR, 1H-NMR and 13C-NMR spectroscopy, PCR) and quantification (HPTLC/HPLC) of the therapeutic secondary metabolites in genetically transformed hairy root cultures (HRCs) induced by Agrobacterium rhizogenes: (i) Anti-cancer pentacyclic triterpenoid ‘Taraxerol’ in HRCs of “Aparajita” (Butterfly pea; (Clitoria ternatea) (ii) ‘Plumbagin’ in HRCs of “Sweta Chitaparu” (White leadwort; Plumbago zeylanica) and (iii) ‘Coumarin’ and ‘β-sitosterol’ in HRCs of “Madanmasta” (Hybanthus enneaspermus) and “Bajramuli’ (Sida acuta).
      • Demonstration of (i) anti-bacterial activity in HRCs of ‘Punarnava’ (Boerhaavia diffusa) against pathogens causing human urinary tract infections (UTI) (ii) anti-oxidative activity and anti-hyperglycaemic activity of a phytosterol purified from S. acuta HRCs in streptozotocin-induced diabetic Wistar albino rats.
      • Comparative multi-elemental profiling by ED-XRF (Energy Dispersive X-ray Fluorescence) and PIXE (Proton Induced X-ray Emission) analysis of in vitro root cultures (non-transformed/Agrobacterium-mediated genetically transformed hairy roots) vis-a-vis natural roots of selected medicinal plants (e.g. A. paniculata, B. diffusa, C. ternatea, P. zeylanica, S. acuta, H. enneaspermus).
      • DNA finger-printing using PCR-based markers (RAPD/ISSR) to generate informative polymorphism for detection of genetic diversity in natural accessions of tea, mango, mungbean, grass pea, cashew nut, and amaranths which will be valuable for marker-assisted selection in breeding programs for these crop species.
      • In vitro clonal multiplication for rapid mass propagation of medicinal & aromatic plant species (“Indian gooseberry” [Emblica officinalis], “Basils” [Ocimum spp.], “Butterfly pea” [Clitoria ternatea], “Curry leaf tree” [Murraya koenigii], “White leadwort” [Plumbago zeylanica], “Madanmastak” [(Hybanthus enneaspermus], ‘‘Kalmegh’ [Andrographis paniculata],  “Indian Privet” [Vitex negundo], “Patherchur” [Coleus ambonicus], “Anantmool“ [Hemidesmus indicus], timber-yielding trees (East Indian rosewood [Dalbergia latifolia], “Shisham” [D. sissoo], and “Sajnapati” [D. lanceolaria], Indian Kino Tree [Pterocarpus marsupium] and cash crops (mulberries, orchids, pomegranate, potato)
      •  Investigating photosynthetic and transpiration responses of in vitro regenerated plants to ex vitro adaptation aimed at optimizing micropropagation protocols to minimize mortality following field transfer.
      • Production of salinity-tolerant palmarosa and lemongrass plants via in vitro salt-selection and their exploitation for reclamation of saline soil at different coastal sites of Odisha while being able to continue as a source of essential aromatic oil of commercial significance.
      • Optimizing parameters for inter-specific somatic hybridization through protoplast fusion in Morus spp (between Japanese cultivars and indigenous cultivars of mulberries), Dalbergia spp. (between timber-yielding D. latifolia/D. sissoo and mangrove species, D. spinosa), Azolla spp. (between high nitrogen-fixing and high biomass-yielding temperate isolates of Azolla filiculoides, A. mexicana or A. caroliniana and temperature-tolerant tropical isolates of Azolla pinnata or A. microphylla) aimed to develop inter-specific somatic hybrid Azolla strains utilizable as efficient biofertilizer for rice cultivation in adverse eco-geographic locations of the Odisha State).
      • Agrobacterium-mediated genetic transformation of grass pea and leafy amaranths (Amaranthus spinosus and A. tricolor) using disarmed Agrobacterium tumefaciens strains carrying binary vectors. Efforts are underway with genetic transformation experiments using specific constructs (human interluken-10) aimed at producing transgenic crop amaranths and mungbean as a dietary source of human immune-modulator protein.
    • Post-doctoral research : (at University of Delhi South Campus, INDIA & University of New Hampshire, Durham, USA: 1998-2000)
      • Transgenic manipulation of polyamine biosynthesis in timber-yielding Dalbergia latifolia and D. sissoo by Agrobacterium/biolistics (gene gun-mediated transfer of Datura/human s-adenosylmethionine decarboxylase (dsamdc / hsamdc) gene constructs towards conferring abiotic stress tolerance
      • Antisense cloning of caffeine synthase gene from tea leaves and efforts for genetic transformation of tea towards production of healthier caffeine-free beverage.
    • Doctoral research : (Ph.D.level: at University of Nottingham, England, UK: 1985-1988)
      • Application of high voltage (500-750 V) - short duration (10-20 μs) electric pulses to freshly isolated protoplasts for stimulation of nucleic acid synthesis concomitant with enhanced plating efficiency and plant regeneration.
      • Realization of totipotency of isolated protoplasts of two medicinal species, Solanum viarum and S. dulcamara and production of spineless protoclonal variants of S. viarum.
      • Development of an improved procedure for fusion of protoplasts between a range of plant species using an optimally buffered and filter-sterilized polyethylene glycol (PEG) preparation with reduced carbonyl content followed by controlled deplasmolysis via hypotonic elution of PEG.
      • Biochemical selection to recover somatic hybrids involving the nitrate reductase-deficient, streptomycin-resistant double mutant (NR–, SR+) tobacco and also via pre-fusion inactivation of protoplasts using antimetabolities (iodoacetamide / sodium iodoacetate, diethylpyrocarbonate, rhodamine 6-G) and/or gamma-irradiation.
      • Flow cytometric characterization and sorting of heterokaryons using an Electronically Programmable Individual Cell Sorter (Coulter EPICS V) following chemical or electrofusion of protoplasts between Solanum viarum and S. dulcamara.
      • Protoplast fusion followed by selection (biochemical / physical) resulting in: (i). Interspecific somatic hybrids between : Solanum viarum (+) S. dulcamara & Lotus corniculatus (+) L. tenuis (ii) Intergeneric somatic hybrids between : Nicotiana tabacum (nitrate reductase deficient, streptomycin resistant double mutant, NR- SR+) (+) S. dulcamara (NR+ SR–)
      • Genetic transformation using Ri plasmid vectors and production of transgenic somaclonal variants of S. Dulcamara
      • Production of transgenic plants of S. dulcamara following: (i) Agrobacterium - mediated genetic transformation of protoplasts (ii) PEG/Electroporation - mediated uptake of recombinant DNA (pCaMVNEO) into protoplasts
  • Additional Responsibilities
    • SL. No. D E S C R I P T I O N TIME FRAME
      1 Director, UGC-Human Resource Development Centre (HRDC), Utkal University (2015- to date)
      2 Course Coordinator, SFS in M.Sc. Environmental science, Utkal University (2012-to date)
      3 Course Coordinator, SFS in M.Sc. Applied Microbiology, Utkal University (2014-to date)
      4 Chairman, Board of Studies in Botany/Applied Microbiology/Environmental Science, Utkal University (2012-to date)
      5 Chairman, S.R.C. in Botany, Utkal University (2012-to date)
      6 Chairman, S.R.C.in Applied Microbiology, Utkal University (2014-to date)
      7 Coordinator, Self Study Report (S.S.R) Preparation Team, Utkal University (w.e.f. 05.08.2015)
      8 Chairman, Environmental Audit, Utkal University (w.e.f. 15.09.2015)
      9 DBT(Department of Biotechnology, Government of India)-nominated External Expert for Institutional Biosafety Committee (IBSC) Of ICAR –National Rice Research Institute (ICAR-NRRI), Cuttack (2016-to date)
      10 Member, Institutional Advisory/Peer Review Committee, Central Research Institute (Ayurveda), Bhubaneswar (Central Council for Research in Ayurveda and Siddha [CCRAS], Department of AYUSH, Ministry of Health & Family Welfare, Govt. of India). (2009-to date)
      11 Member , Scientific Advisory Committee, Regional Plant Resource Centre (RPRC), Department of Environment & Forests, Government of Odisha. (2013-to date)
      12 Member, Expert Committee to examine the Access and Benefit Sharing Applications, Odisha Biodiversity Board, Department of Environment & Forests, Government of Odisha (2015 -to date)
      13 Member, Academic Council, Salipur Autonomous College, Salipur, Odisha (2014-to date)
      14 Member, Academic Council, Nayagarh Autonomous College, Nayagarh, Odisha (2016-to date)
      15 Member, University Research Committee (URC), Utkal University (2014-to date)
      16 Member, College Development Council (CDC),Utkal University (2014-to date)
      17 Member, Research and Development (R&D) Committee, Utkal University (2009-2014)
      18 Member, Library committee, Utkal University (2015-to date)
      19 Member, Admission Committee, Utkal University (2014-to date)
      20 Member, Internal Quality Assurance Cell (IQAC), Utkal University (2014-to date)
      21 Member, Squad Committee on Maintenance of Law and Order, Utkal University (2015-to date)
      22 Member, Scrutiny Committee For Faculty Recruitment, Utkal University (2015-to date)
      23 Member, Garden Committee, Utkal University (2015-to date)
      24 Co-ordinator, UGC-sponsored DRS Programme in Botany (SAP, Phase-I) , Utkal University (2002-2007)
      25 Managing Editor, “Plant Science Research”, Journal of the Orissa Botanical Society (2009-2011)
      26 President, Old Students’ Association, P.G. Department of Botany, Utkal University (2009-to date)